identification of excretory-secretory products from larval stages of ostertagia circumcincta cultured in vitro by sds-page and immunoblotting

exsheathing fluid (ef) and excretory-secretory products (es) of infective third-stage cultured larvae ofostertagia circumcincta were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sdspage). five and seven predominant proteins were found in the ef and es products, respectively. immunoblotting by sheep pre-infection serum did not react with any of the ef and es proteins, but the postinfection serum recognized four proteins of 44.5, 41.5, 38 and 24 kda of the es products. none of the ef products was recognized by the post-infection serum. protectivity of the four proteins remains to be determined.